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43-495 (1ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-495 (5ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
43-495 (1ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HOXB6 Antibody

CATALOG NUMBER: 43-495

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: EPRKSDCAQDKS
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Approx 26kDa band observed in lysates of cell lines HEK293, CACO-2 and MRC5 PD19 (calculated MW of 25.4kDa according to NP_061825.2). Recommended concentration: 1-3ug/ml.Immunohistochemistry:In paraffin embedded Human Lung shows nuclear staining in the alvioli. Recommended concentration, 5-10ug/ml.
Predicted Molecular Weight:
Approx 26kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
HOXB6
Additional Names:
HOXB6, homeobox B6, HOX2, HOX2B, HU-2, Hox-2.2, homeo box 2B, homeo box B6, homeobox protein Hox-2.2, homeobox protein Hox-2B, homeobox protein Hox-B6, homeobox protein Hu-2
Protein Accession Number:
NP_061825.2
Protein Gi Number:
23503237
Ncbi Gene Id Number:
3216
Background Reference 1:
Ferrai C, Naum-Onganía G, Longobardi E, Palazzolo M, Disanza A, Diaz VM, Crippa MP, Scita G, Blasi F.Induction of HoxB transcription by retinoic acid requires actin polymerization.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-495

List Size:
0.1 mg

List Price:

$469.00

Out of stock

Datasheet
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