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Fluorescent image of Hela cell stained with HOXC10 Antibody .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HOXC10 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml).HOXC10 immunoreactivity is localized to Nucleus significantly.
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).
Fluorescent image of Hela cell stained with HOXC10 Antibody .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HOXC10 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml).HOXC10 immunoreactivity is localized to Nucleus significantly.

HOXC10 Antibody

CATALOG NUMBER: 60-864

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This HOXC10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 224-250 amino acids from the C-terminal region of human HOXC10.
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
For IF starting dilution is: 1:10~50
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
38 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HOXC10
Additional Names:
Homeobox protein Hox-C10, Homeobox protein Hox-3I, HOXC10, HOX3I
Protein Accession Number:
Q9NYD6
Ncbi Gene Id Number:
3226
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene belongs to the homeobox family of genes. Thehomeobox genes encode a highly conserved family of transcriptionfactors that play an important role in morphogenesis in allmulticellular organisms. Mammals possess four similar homeobox geneclusters, HOXA, HOXB, HOXC and HOXD, which are located on differentchromosomes and consist of 9 to 11 genes arranged in tandem. Thisgene is one of several homeobox HOXC genes located in a cluster onchromosome 12. The protein level is controlled during celldifferentiation and proliferation, which may indicate this proteinhas a role in origin activation.
Background Reference 1:
Hwang, J.H., et al. Cloning Stem Cells 11(2):269-279(2009)
Background Reference 2:
Zhai, Y., et al. Cancer Res. 67(21):10163-10172(2007)
Background Reference 3:
Gabellini, D., et al. EMBO J. 22(14):3715-3724(2003)
Background Reference 4:
Kosaki, K., et al. Teratology 65(2):50-62(2002)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-864

List Size:
400 ul

List Price:

$495.00

Datasheet
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