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43-397 (0.3ug/ml) staining of Human Olfactory bulb lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-397 (0.01ug/ml) staining of Mouse (A) and Rat (B) Brain lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-397 (0.03ug/ml) staining of Pig Brain lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-397 (5ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
43-397 (0.3ug/ml) staining of Human Olfactory bulb lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

HOXC6, Antibody

CATALOG NUMBER: 43-397

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Pig, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Pig, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: C-PNVALNSTAYDPVRH
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 26kDa band observed in Human Brain (Olfactory bulb) and in Rat and Pig Brain lysates (calculated MW of 26.9kDa according to NP_004494.1). In Mouse Brain lysates the band runs like a 27kDa band. Recommended concentration: 0.03-0.1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Tonsil. Recommended concentration: 5ug/ml.
Specificity:
This antibody is expected to recognize isoform 1 (NP_004494.1) only.
Predicted Molecular Weight:
Approx 26kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
HOXC6
Additional Names:
HOXC6, homeobox C6, CP25, HHO.C8, HOX3, HOX3C, homeo box 3C, homeo box C6, homeo box C8 protein, homeobox protein CP25, homeobox protein HHO.C8, homeobox protein Hox-3C, homeobox protein Hox-C6
Protein Accession Number:
NP_004494.1
Protein Gi Number:
4758554
Ncbi Gene Id Number:
3223
Background Reference 1:
Ansari KI, Hussain I, Shrestha B, Kasiri S, Mandal SS.HOXC6 Is transcriptionally regulated via coordination of MLL histone methylase and estrogen receptor in an estrogen environment.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-397

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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