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Fluorescent image of HUVEC cell stained with HOXD8 Antibody .HUVEC cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HOXD8 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml).HOXD8 immunoreactivity is localized to Nucleus significantly.
Western blot analysis in T47D cell line lysates (35ug/lane).
Fluorescent image of HUVEC cell stained with HOXD8 Antibody .HUVEC cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HOXD8 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml).HOXD8 immunoreactivity is localized to Nucleus significantly.

HOXD8 Antibody

CATALOG NUMBER: 60-085

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This HOXD8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 243-270 amino acids from the C-terminal region of human HOXD8.
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
For IF starting dilution is: 1:10~50
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
32 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HOXD8
Additional Names:
Homeobox protein Hox-D8, Homeobox protein Hox-4E, Homeobox protein Hox-54, HOXD8, HOX4E
Protein Accession Number:
P13378
Protein Gi Number:
13124737
Ncbi Gene Id Number:
3234
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene belongs to the homeobox family of genes. Thehomeobox genes encode a highly conserved family of transcriptionfactors that play an important role in morphogenesis in allmulticellular organisms. Mammals possess four similar homeobox geneclusters, HOXA, HOXB, HOXC and HOXD, located on differentchromosomes, consisting of 9 to 11 genes arranged in tandem. Thisgene is one of several homeobox HOXD genes located in a cluster onchromosome 2. Deletions that remove the entire HOXD gene cluster orthe 5' end of this cluster have been associated with severe limband genital abnormalities. In addition to effects duringembryogenesis, this particular gene may also play a role in adulturogenital tract function.
Background Reference 1:
Ester, A.R., et al. Am. J. Med. Genet.149A (12), 2745-2752 (2009) :
Background Reference 2:
Yerges, L.M., et al. J. Bone Miner. Res. 24(12):2039-2049(2009)
Background Reference 3:
Harada, K., et al. J. Cell. Sci. 122 (PT 21), 3923-3930 (2009) :
Background Reference 4:
Goodman, F.R. Am. J. Med. Genet. 112(3):256-265(2002)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-085

List Size:
400 ul

List Price:

$495.00

Datasheet
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