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Western blot analysis of extracts of various cell lines, using HPCAL1 antibody (13-378) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunoprecipitation analysis of extracts of HepG2 cells using HPCAL1 antibody (13-378). Western blot was performed from the immunoprecipitate using HPCAL1 antibody (13-378) at a dilition of 1:1000.
Western blot analysis of extracts of various cell lines, using HPCAL1 antibody (13-378) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

HPCAL1 Antibody

CATALOG NUMBER: 13-378

Clonality:
Polyclonal
Tested Applications:
IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-193 of human HPCAL1 (NP_002140.2).
Conjugate:
Unconjugated
Tested Applications:
IP, WB
Application Note:
WB: 1:500 - 1:2000
IP: 1:20 - 1:100
Positive Control 1:
SH-SY5Y
Positive Control 2:
HepG2
Positive Control 3:
HT-1080
Positive Control 4:
NCI-H460
Positive Control 5:
HeLa
Positive Control 6:
Mouse brain
Predicted Molecular Weight:
Observed: 17kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
HPCAL1
Additional Names:
BDR1, HLP2, VILIP-3, hippocalcin-like protein 1, calcium-binding protein BDR-1, visinin-like protein 3
Ncbi Gene Id Number:
3241
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a member of neuron-specific calcium-binding proteins family found in the retina and brain. It is highly similar to human hippocalcin protein and nearly identical to the rat and mouse hippocalcin like-1 proteins. It may be involved in the calcium-dependent regulation of rhodopsin phosphorylation and may be of relevance for neuronal signalling in the central nervous system. Several alternatively spliced transcript variants encoding the same protein have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-378

List Size:
100 uL

List Price:

$436.00

Datasheet
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