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Western blot analysis of extracts of various cell lines, using HPGD antibody (22-560) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat intestine using HPGD antibody (22-560) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using HPGD antibody (22-560) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using HPGD antibody (22-560) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

HPGD Antibody

CATALOG NUMBER: 22-560

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-266 of human HPGD (NP_000851.2).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
SW480
Positive Control 2:
Jurkat
Positive Control 3:
Mouse liver
Positive Control 4:
Mouse lung
Positive Control 5:
Mouse spleen
Predicted Molecular Weight:
Observed: 29kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
HPGD
Additional Names:
HPGD, PGDH1, 15-PGDH, hydroxyprostaglandin dehydrogenase 15-(NAD), Prostaglandin dehydrogenase 1
Ncbi Gene Id Number:
3248
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the short-chain nonmetalloenzyme alcohol dehydrogenase protein family. The encoded enzyme is responsible for the metabolism of prostaglandins, which function in a variety of physiologic and cellular processes such as inflammation. Mutations in this gene result in primary autosomal recessive hypertrophic osteoarthropathy and cranioosteoarthropathy. Multiple transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-560

List Size:
100 uL

List Price:

$436.00

Datasheet
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