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45-070 (0.1ug/ml) staining of Human Duodenum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-070 (2.5ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-070 (0.1ug/ml) staining of Human Duodenum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HPGD Antibody

CATALOG NUMBER: 45-070

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-DYDTTPFQAKTQ
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Experiments gave bands at approx 26kDa and 50kDa in Human Duodenum lysates after 0.1ug/ml antibody staining. These bands correspond to earlier findings in literature with different antibodies (PMID: 11889207). This protein has a calculated MW of 29.0kDa according to NP_000851. Recommended concentration: 0.1-1ug/ml.Immunohistochemistry:Paraffin embedded Human Colon and Liver. Recommended concentration: 2.5µg/ml.
Positive Control 1:
Cat. No. 1364 - Human Duodenum Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
HPGD
Additional Names:
HPGD, Prostaglandin dehydrogenase 1, PGDH1, 15-PGDH, hydroxyprostaglandin dehydrogenase 15-(NAD), PGDH, SDR36C1, NAD+-dependent 15-hydroxyprostaglandin dehydrogenase, short chain dehydrogenase/reductase family 36C, member 1
Protein Accession Number:
NP_000851.2
Protein Gi Number:
31542939
Ncbi Gene Id Number:
3248
Background Reference 1:
Coggins KG, Latour A, Nguyen MS, Audoly L, Coffman TM, Koller BH. Metabolism of PGE2 by prostaglandin dehydrogenase is essential for remodeling the ductus arteriosus. Nat Med. 2002 Feb;8(2):91-2. No abstract available.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-070

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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