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Western Blot at 1:2000 dilution + human placenta lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of HPGD Antibody in MDA-MB231 cell line lysates (35ug/lane)
HPGD Antibody IHC analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western Blot at 1:2000 dilution + human placenta lysate Lysates/proteins at 20 ug per lane.

HPGD Antibody

CATALOG NUMBER: 62-470

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Monkey
Immunogen:
This HPGD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 184-212 amino acids from the C-terminal region of human HPGD.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
29 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HPGD
Additional Names:
15-hydroxyprostaglandin dehydrogenase [NAD(+)], 15-PGDH, Prostaglandin dehydrogenase 1, HPGD, PGDH1
Protein Accession Number:
P15428
Protein Gi Number:
129889
Ncbi Gene Id Number:
3248
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
HPGD is a member of the short-chain nonmetalloenzyme alcohol dehydrogenase protein family. This protein is responsible for the metabolism of prostaglandins, which function in a variety of physiologic and cellular processes such as inflammation.
Background Reference 1:
Thill,M., et.al., Anticancer Res. 29 (9), 3619-3625 (2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-470

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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