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Western blot analysis using anti - human HPRT monoclonal antibody against truncated HPRT recombinant protein.
Western blot analysis using anti - human HPRT monoclonal antibody against truncated HPRT recombinant protein.

HPRT Antibody [1F8D11]

CATALOG NUMBER: 32-170

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Ni-NTA purified truncated recombinant HPRT expressed in E. Coli strain BL21 (DE3).
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Western Blot:Dilution 1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Clonality:
Monoclonal
Isotype:
IgG2b
Physical State:
Liquid
Concentration:
batch dependent
Storage Conditions:
HPRT monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HPRT1
Additional Names:
HPRT, Hypoxanthine-guanine phosphoribosyltransferase, HGPRT
Protein Accession Number:
P00492
Protein Gi Number:
123497
Ncbi Gene Id Number:
3251
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The HPRT1 gene provides instructions for making an enzyme called hypoxanthine phosphoribosyltransferase 1. This enzyme allows cells to recycle purines, some of the building blocks of DNA and its chemical cousin RNA.The enzyme hypoxanthine-guanine phosphoribosyltrasferase (E.C.2.4.2.8., HPRT) plays a crucial role in uric acid synthesis and purine metabolism.This enzyme catalyzes the conversion of hypoxanthine and guanine to inosine monophosphate (IMP) and guanosine monophosphate (GMP), respectively, and uses phosphoribosylpyrophosphate (PRPP) as a cosubstrate and as a source of energy . This pathway is also known as the purine salvage pathway because it allows cells to reuse purine compounds to build DNA and RNA.
Background Reference 1:
Manjanatha MG, et.al Mutat Res. 2004 Mar 22;547(1-2):5-18.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

32-170

List Size:
0.1 mL

List Price:

$408.00

Datasheet
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