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Western blot analysis of HPRT1 antibody in Hela cell line lysates (35ug/lane)
Western blot analysis of HPRT1 using rabbit polyclonal HPRT1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HPRT1 gene.
HPRT1 Antibody IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of HPRT1 antibody in Hela cell line lysates (35ug/lane)

HPRT1 Antibody

CATALOG NUMBER: 62-435

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Monkey
Immunogen:
This HPRT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 150-178 amino acids from the C-terminal region of human HPRT1.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
25 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HPRT1
Additional Names:
Hypoxanthine-guanine phosphoribosyltransferase, HGPRT, HGPRTase, HPRT1, HPRT
Protein Accession Number:
P00492
Protein Gi Number:
123497
Ncbi Gene Id Number:
3251
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway.
Background Reference 1:
Ceballos-Picot,I., Hum. Mol. Genet. 18 (13), 2317-2327 (2009)
Background Reference 2:
Sculley,D.G., Hum. Genet. 90 (3), 195-207 (1992)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-435

List Size:
400 ul

List Price:

$495.00

Datasheet
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