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Formalin-fixed, paraffin-embedded human cervix stained with HPV-16 antibody (SPM405)
Formalin-fixed, paraffin-embedded human cervix stained with HPV-16 antibody (SPM405)

HPV-16 Antibody [SPM405]

CATALOG NUMBER: 33-524

Clonality:
Monoclonal
Tested Applications:
IF, IHC-P
Host Species:
Mouse
Species Reactivity:
HPV-16
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
HPV-16
Immunogen:
Recombinant major capsid protein L1 from human papilloma virus type 16 was used as the immunogen for the HPV-16 antibody.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P
Application Note:
Immunofluorescence: 0.5-1 ug/ml
Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)
Optimal dilution of the HPV-16 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG2a, kappa
Physical State:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
L1
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
Reacts with a protein of 57kDa, identified as the L1 protein of human papilloma virus type 16 (HPV-16). It is the major capsid protein of HPV-16. Infection with specific types of HPV has been associated with an increased risk of developing cervical neoplasia. HPV types 6 and 11 have been associated with relatively benign diseases such as genital warts but types 16 and 18 are strongly associated with cervical, vaginal, and vulvar malignancies. The antibody reacts very strongly with formalin-fixed, paraffin-embedded tissues containing HPV-16 or -33; very weak reactions were occasionally observed with biopsy specimens or smears containing HPV-6 or HPV-11. It cross-reacts with HPV37.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-524

List Size:
100 ug

List Price:

$626.00

Datasheet
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