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46-855 (5ug/ml) staining of paraffin embedded Human Liver. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
46-855 (5ug/ml) staining of paraffin embedded Human Liver. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

HRAS-like Suppressor 2 Antibody

CATALOG NUMBER: 46-855

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: QELPYSLTSDNC
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx. 21kDa band in Human Kidney lysates after 0.5 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 17.4kDa according to NP_060348.1 (provisional). The 21kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Liver shows a granular pattern in the cytoplasm of hepatocytes. Recommended concentration, 5-10ug/ml.
Specificity:
This design was based on provisional sequence data.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
HRASLS2
Additional Names:
HRASLS2 , HRAS-like suppressor 2, FLJ20556
Protein Accession Number:
NP_060348.1
Protein Gi Number:
8923526
Ncbi Gene Id Number:
54979
Background Reference 1:
Shyu RY, Hsieh YC, Tsai FM, Wu CC, Jiang SY. Cloning and functional characterization of the HRASLS2 gene. Amino Acids. 2008 Jun;35(1):129-37.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-855

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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