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Western blot analysis in ZR-75-1 cell line lysates (35ug/lane).
HRC Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in ZR-75-1 cell line lysates (35ug/lane).

HRC Antibody

CATALOG NUMBER: 56-609

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This HRC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 277-306 amino acids from the Central region of human HRC.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
80 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HRC
Additional Names:
Sarcoplasmic reticulum histidine-rich calcium-binding protein, HRC, HCP
Protein Accession Number:
P23327
Protein Gi Number:
134873
Ncbi Gene Id Number:
3270
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a luminal sarcoplasmic reticulum proteinidentified by its ability to bind low-density lipoprotein with highaffinity. The protein interacts with the cytoplasmic domain oftriadin, the main transmembrane protein of the junctionalsarcoplasmic reticulum (SR) of skeletal muscle. The proteinfunctions in the regulation of releasable calcium into the SR.
Background Reference 1:
Arvanitis, D.A., et al. Eur. Heart J. 29(20):2514-2525(2008)
Background Reference 2:
Arvanitis, D.A., et al. Am. J. Physiol. Heart Circ. Physiol. 293 (3), H1581-H1589 (2007) :
Background Reference 3:
Fan, G.C., et al. Am. J. Physiol. Heart Circ. Physiol. 287 (4), H1705-H1711 (2004) :
Background Reference 4:
Anderson, J.P., et al. Mol. Cell. Biol. 24(9):3757-3768(2004)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-609

List Size:
400 ul

List Price:

$495.00

Datasheet
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