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Western blot analysis in CEM cell line lysates (35ug/lane).
HRH1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in CEM cell line lysates (35ug/lane).

HRH1 Antibody

CATALOG NUMBER: 57-725

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This HRH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 273-301 amino acids from the Central region of human HRH1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
56 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HRH1
Additional Names:
Histamine H1 receptor, H1R, HH1R, HRH1
Protein Accession Number:
P35367
Protein Gi Number:
547645
Ncbi Gene Id Number:
3269
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Histamine is a ubiquitous messenger molecule released frommast cells, enterochromaffin-like cells, and neurons. Its variousactions are mediated by histamine receptors H1, H2, H3 and H4. Thisgene was thought to be intronless until recently. The proteinencoded by this gene is an integral membrane protein and belongs tothe G protein-coupled receptor superfamily. It mediates thecontraction of smooth muscles, the increase in capillarypermeability due to contraction of terminal venules, the release ofcatecholamine from adrenal medulla, and neurotransmission in thecentral nervous system. Multiple alternatively spliced variants,encoding the same protein, have been identified. [provided byRefSeq].
Background Reference 1:
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background Reference 2:
Ruano, G., et al. Pharmacogenomics 11(7):959-971(2010)
Background Reference 3:
Davila, S., et al. Genes Immun. 11(3):232-238(2010)
Background Reference 4:
Notcovich, C., et al. Exp. Cell Res. 316(3):401-411(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-725

List Size:
400 ul

List Price:

$495.00

Datasheet
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