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Western blot analysis of extracts of normal 293T cells, 293T transfected with EPCAM-His and 293T transfected with NPPA-His Protein, using HRP-conjugated Rabbit anti His-Tag mAb at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 0.09s.
Western blot analysis of extracts of normal 293T cells, 293T transfected with EPCAM-His and 293T transfected with NPPA-His Protein, using HRP-conjugated Rabbit anti His-Tag mAb at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 0.09s.

HRP-conjugated Rabbit anti His-Tag Recombinant Antibody (84-528)

CATALOG NUMBER: 84-528

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Species independent
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Species independent
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
WB,1:2000 - 1:10000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG2B,Kappa
Physical State:
Liquid
Buffer:
Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
His
Additional Names:
His;His tag;His-tag
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected by using His antibodies.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

84-528

List Size:
100 uL

List Price:

$204.00

Datasheet
Shipping Info

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