Dose response curve of HRP conjugated Rabbit Anti-Human IgE (Fc) mAb measured by ELISA. 1 µg/mL of various immunoglobulins were coated to 384-well plate., blank wells without protein were used as negative control (NC). The coated plate was blocked and subsequently incubated with 25 µL of HRP conjugated Rabbit Anti-Human IgE (Fc) mAb in a 2 fold serial dilution from 2 μg/mL to 6.1×10^-5 pg/mL, incubation was performed at room temperature for 1 hour. The ELISA result demonstrated that Rabbit Anti-Human IgE (Fc) mAb has highly specific recognition of Human IgE while no or minimal cross reactivity to Human IgG1、Human IgG2、Human IgG3、Human IgG4、Human IgM、Mouse IgG、Rat IgG.