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Western blot analysis of recombinan Human IgG1 Kappa using HRP-conjugated Rabbit anti-Human Kappa Light Chain mAb at 1:500-1:32000 dilution incubated overnight at 4℃. | Lysates/proteins: 100ng μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 5s.
Western blot analysis of recombinan Human IgG1kappa,Human IgG2κ,Human IgG4κ using HRP-conjugated Rabbit anti-Human Kappa Light Chain mAbat 1:1000 dilution incubated overnight at 4℃. | Lysates/proteins: 100ng μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Negative control (NC): Human IgG1λ,Human IgG2λ | Exposure time: 5s.
Immunohistochemistry analysis of paraffin-embedded Human appendix tissue using HRP-conjugated Rabbit anti-Human Kappa Light Chain mAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using HRP-conjugated Rabbit anti-Human Kappa Light Chain mAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Dose response curve of HRP conjugated Rabbit Anti-Human Kappa Light Chain mAb measured by ELISA. 1 µg/mL of various immunoglobulins were coated to 384-well plate., blank wells without protein were used as negative control (NC). The coated plate was blocked and subsequently incubated with 25 µL of HRP conjugated Rabbit Anti-Human Kappa Light Chain mAb in a 2 fold serial dilution from 2 μg/mL to 6.1×10^-5 pg/mL, incubation was performed at room temperature for 1 hour. The ELISA result demonstrated that Rabbit anti-Human Kappa Light Chain mAb has highly specific recognition of Human IgG1, κ、Human IgG2, κ、Human IgG4, κ、 Human IgM, κ，while no or minimal cross reactivity to Human IgG1, λ、Human IgG2, λ、Human IgG3, λ、Rat IgG1, λ、Mouse IgG3, κ.
Western blot analysis of recombinan Human IgG1 Kappa using HRP-conjugated Rabbit anti-Human Kappa Light Chain mAb at 1:500-1:32000 dilution incubated overnight at 4℃. | Lysates/proteins: 100ng μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 5s.

HRP-conjugated Rabbit anti-Human Kappa Light Chain Recombinant Antibody (84-788)

CATALOG NUMBER: 84-788

Clonality:
Monoclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
HRP
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This information is considered to be commercially sensitive.
Conjugate:
HRP
Tested Applications:
ELISA, IHC-P, WB
Application Note:
WB,1:5000-1:10000, IHC-P,1:50-1:200, ELISA,1:5000-1:10000
Clonality:
Monoclonal
Physical State:
Liquid
Buffer:
Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
Human Immunoglobulin kappa constant
User Note:
Optimal dilutions for each application to be determined by the researcher.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

84-788

List Size:
100 uL

List Price:

$87.00

Datasheet
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