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Western blot analysis of HS3ST2 Antibody in Hela, 293 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human brain tissue reacted with HS3ST2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of HS3ST2 Antibody in Hela, 293 cell line lysates (35ug/lane)

HS3ST2 Antibody

CATALOG NUMBER: 63-982

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This HS3ST2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 317-346 amino acids from the C-terminal region of human HS3ST2.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
42 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HS3ST2
Additional Names:
Heparan sulfate glucosamine 3-O-sulfotransferase 2, Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 2, 3-OST-2, Heparan sulfate 3-O-sulfotransferase 2, h3-OST-2, HS3ST2, 3OST2
Protein Accession Number:
Q9Y278
Protein Gi Number:
61214416
Ncbi Gene Id Number:
9956
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Heparan sulfate biosynthetic enzymes are key components in generating a myriad of distinct heparan sulfate fine structures that carry out multiple biologic activities. The enzyme is a member of the heparan sulfate biosynthetic enzyme family. It is a type II integral membrane protein and possesses heparan sulfate glucosaminyl 3-O-sulfotransferase activity.
Background Reference 1:
Ikeda, M., et al. Biol. Psychiatry 67(3):263-269(2010)
Background Reference 2:
Nat. Genet. 41(7):824-828(2009)
Background Reference 3:
Mochizuki, H., et al. J. Biol. Chem. 283(45):31237-31245(2008)
Background Reference 4:
Lawrence, R., et al. Matrix Biol. 26(6):442-455(2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-982

List Size:
400 ul

List Price:

$495.00

Datasheet
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