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Antibody used in WB on Human MCF-7 at 0.2-1 ug/ml.
Antibody used in WB on Human MCF-7 at 0.2-1 ug/ml.

HS3ST5 Antibody

CATALOG NUMBER: 30-503

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HS3ST5.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
HS3ST5 antibody can be used for detection of HS3ST5 by ELISA at 1:1562500. HS3ST5 antibody can be used for detection of HS3ST5 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1219 - MCF7 Cell Lysate
Predicted Molecular Weight:
40 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store HS3ST5 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
HS3ST5
Additional Names:
HS3ST5, 3-OST-5, 3OST5, HS3OST5, NBLA04021
Protein Accession Number:
NP_705840
Protein Gi Number:
45267824
Ncbi Gene Id Number:
222537
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
HS3ST5 is the rate limiting enzyme for synthesis of HSact. It performs the crucial step modification in the biosynthesis of anticoagulant heparan sulfate (HSact) that is to complete the structure of the antithrombin pentasaccharide binding site. HS3ST5 also generates GlcUA-GlcNS or IdoUA-GlcNS and IdoUA2S-GlcNH2. The substrate-specific O-sulfation generates an enzyme-modified heparan sulfate which acts as a binding receptor to Herpes simplex virus-1 (HSV-1) and permits its entry.HS3ST5 belongs to a group of heparan sulfate 3-O-sulfotransferases (EC 2.8.2.23) that transfer sulfate from 3-prime-phosphoadenosine 5-prime phosphosulfate (PAPS) to heparan sulfate and heparin (Mochizuki et al., 2003 [PubMed 12740361]).
Background Reference 1:
Chen, J. (2005) Biochim. Biophys. Acta 1725 (2), 190-200.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

30-503

List Size:
100 ul

List Price:

$519.00

Datasheet
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