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Western blot analysis of extracts of various cell lines, using Hsc70 antibody (13-851).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human tonsil using Hsc70 antibody (13-851) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human mammary cancer using Hsc70 antibody (13-851) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of NIH-3T3 cells using Hsc70 antibody (13-851).
Immunofluorescence analysis of MCF-7 cells using Hsc70 antibody (13-851).
Western blot analysis of extracts of various cell lines, using Hsc70 antibody (13-851).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Hsc70 Antibody [31H6]

CATALOG NUMBER: 13-851

Clonality:
Monoclonal
Tested Applications:
IF, IHC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein of human Hsc70
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, IP, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
IP: 1:20 - 1:50
Positive Control 1:
HepG2
Positive Control 2:
HeLa
Positive Control 3:
NIH/3T3
Positive Control 4:
PC-12
Predicted Molecular Weight:
Observed: 71kDa
Purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
HSPA8
Additional Names:
HSPA8, heat shock 70 kDa protein 8, HSC54, HSC70, HSC71, HSP71, HSP73, HSPA10, LAP, MGC131511, MGC29929, NIP71, LPS-associated protein 1, N-myristoyltransferase inhibitor protein 71, constitutive heat shock protein 70, heat shock 70kD protein 8, heat shock 70kd protein 10, heat shock cognate protein 54, heat shock cognate protein, 71- kDa, lipopolysaccharide-associated protein, uncharacterized bone marrow protein BM034
Ncbi Gene Id Number:
3312
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-851

List Size:
50 uL

List Price:

$365.00

Datasheet
Shipping Info

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