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45-748 (0.3ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-748 (0.3ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HSD11B1 Antibody

CATALOG NUMBER: 45-748

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Cow
Immunogen:
The immunogen for this antibody is: CTSYNMDRFINK
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 35kDa band observed in Human Liver lysates (calculated MW of 32.4kDa according to NP_005516.1 and NP_861420.1). Recommended concentration: 0.3-1ug/ml.
Specificity:
This antibody is expected to recognise human HSD11B1 protein. Both NP_005516.1 and NP_861420.1 variants encode the same protein.
Positive Control 1:
Cat. No. 1304 - Human Liver Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
HSD11B1
Additional Names:
HSD11B1, HDL, 11-DH, HSD11L, MGC13539, 11-beta-HSD1, hydroxysteroid (11-beta) dehydrogenase 1, corticosteroid 11-beta-dehydrogenase isozyme 1, HSD11, HSD11B, SDR26C1, 11-beta-hydroxysteroid dehydrogenase 1, OTTHUMP00000034650, short chain dehydrogenase/reductase family 26C, member 1
Protein Accession Number:
NP_005516.1,NP_861420.1
Protein Gi Number:
5031765
Ncbi Gene Id Number:
3290
Background Reference 1:
Paterson JM, Morton NM, Fievet C, Kenyon CJ, Holmes MC, Staels B, Seckl JR, Mullins JJ. Metabolic syndrome without obesity: Hepatic overexpression of 11beta-hydroxysteroid dehydrogenase type 1 in transgenic mice. Proc Natl Acad Sci USA. 2004 May 4;101(18):7088-93. Epub 2004 Apr 26.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-748

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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