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Western blot analysis of extracts of various cell lines, using HSD17B10 antibody (19-669) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Western blot analysis of extracts of various cell lines, using HSD17B10 antibody (19-669) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human tonsil using HSD17B10 antibody (19-669) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using HSD17B10 antibody (19-669) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using HSD17B10 antibody (19-669) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U-2 OS cells using HSD17B10 Polyclonal Antibody (19-669) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using HSD17B10 antibody (19-669) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

HSD17B10 Antibody

CATALOG NUMBER: 19-669

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-261 of human HSD17B10 (NP_004484.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
IF: 1:50 - 1:100
Positive Control 1:
Lovo
Positive Control 2:
293T
Positive Control 3:
HepG2
Positive Control 4:
BT-474
Positive Control 5:
22Rv1
Positive Control 6:
SH-SY5Y
Predicted Molecular Weight:
Observed: 26kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
HSD17B10
Additional Names:
HADH2, ABAD, ERAB, MHBD, HSD17B10, 17b-HSD10, hydroxyacyl-Coenzyme A dehydrogenase, type II, type 10 17b-HSD, AB-binding alcohol dehydrogenase, type 10 17beta-hydroxysteroid dehydrogenase, HADH2, MRPP2, SCHAD, XH98G2
Ncbi Gene Id Number:
3028
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes 3-hydroxyacyl-CoA dehydrogenase type II, a member of the short-chain dehydrogenase/reductase superfamily. The gene product is a mitochondrial protein that catalyzes the oxidation of a wide variety of fatty acids and steroids, and is a subunit of mitochondrial ribonuclease P, which is involved in tRNA maturation. The protein has been implicated in the development of Alzheimer disease, and mutations in the gene are the cause of 17beta-hydroxysteroid dehydrogenase type 10 (HSD10) deficiency. Several alternatively spliced transcript variants have been identified, but the full-length nature of only two transcript variants has been determined.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-669

List Size:
100 uL

List Price:

$436.00

Datasheet
Shipping Info

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