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Western blot analysis in A431,SiHa cell line lysates (35ug/lane).
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with HSD17B12 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in A431,SiHa cell line lysates (35ug/lane).

HSD17B12 Antibody

CATALOG NUMBER: 63-906

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Monkey
Immunogen:
This HSD17B12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 126-155 amino acids from the Central region of human HSD17B12.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
34 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HSD17B12
Additional Names:
Estradiol 17-beta-dehydrogenase 12, 17-beta-hydroxysteroid dehydrogenase 12, 17-beta-HSD 12, 3-ketoacyl-CoA reductase, KAR, 131-, HSD17B12
Protein Accession Number:
Q53GQ0
Protein Gi Number:
158931120
Ncbi Gene Id Number:
51144
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The enzyme 17-beta hydroxysteroid dehydrogenase-12 (HSD17B12) uses NADPH to reduce 3-ketoacyl-CoA to 3-hydroxyacyl-CoA during the second step of fatty acid elongation.
Background Reference 1:
Plourde,M. J. Steroid Biochem. Mol. Biol. 116 (3-5), 134-153 (2009)
Background Reference 2:
Nagasaki,S. Mol. Cell. Endocrinol. 307 (1-2), 163-168 (2009)
Background Reference 3:
Bellemare,V. J. Steroid Biochem. Mol. Biol. 114 (3-5), 129-134 (2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-906

List Size:
400 ul

List Price:

$495.00

Datasheet
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