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Western Blot at 1:1000 dilution + MDA-MB-453 whole cell lysate Lysates/proteins at 20 ug per lane.
HSD17B2 Antibody IHC analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western Blot at 1:1000 dilution + MDA-MB-453 whole cell lysate Lysates/proteins at 20 ug per lane.

HSD17B2 Antibody

CATALOG NUMBER: 61-801

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This HSD17B2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 265-294 amino acids from the Central region of human HSD17B2.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
43 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HSD17B2
Additional Names:
Estradiol 17-beta-dehydrogenase 2, 17-beta-hydroxysteroid dehydrogenase type 2, 17-beta-HSD 2, 20 alpha-hydroxysteroid dehydrogenase, 20-alpha-HSD, E2DH, Microsomal 17-beta-hydroxysteroid dehydrogenase, Testosterone 17-beta-dehydrogenase, HSD17B2, EDH17B2
Protein Accession Number:
P37059
Protein Gi Number:
544152
Ncbi Gene Id Number:
3294
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
HSD17B2 is capable of catalyzing the interconversion of testosterone and androstenedione, as well as estradiol and estrone.HSD17B2 also has 20-alpha-HSD activity. HSD17B2 uses NADH while EDH17B3 uses NADPH.
Background Reference 1:
Shen, Z., et al. Endocrinology 150(11):4941-4949(2009)
Background Reference 2:
Bhavani, V., et al. Cancer Biomark 5(4):207-213(2009)
Background Reference 3:
Olsen, J.V., et al. Cell 127(3):635-648(2006)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-801

List Size:
400 ul

List Price:

$495.00

Datasheet
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