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Western Blot at 1:1000 dilution Lane 1: human testis lysate Lane 2: Raji whole cell lysate Lysates/proteins at 20 ug per lane.
Formalin-fixed and paraffin-embedded human testis tissue reacted with HSD17B3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western Blot at 1:1000 dilution Lane 1: human testis lysate Lane 2: Raji whole cell lysate Lysates/proteins at 20 ug per lane.

HSD17B3 Antibody

CATALOG NUMBER: 63-983

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This HSD17B3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-118 amino acids from the Central region of human HSD17B3.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
35 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HSD17B3
Additional Names:
Testosterone 17-beta-dehydrogenase 3, 17-beta-hydroxysteroid dehydrogenase type 3, 17-beta-HSD 3, Testicular 17-beta-hydroxysteroid dehydrogenase, HSD17B3, EDH17B3
Protein Accession Number:
P37058
Protein Gi Number:
1169300
Ncbi Gene Id Number:
3293
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This isoform of 17 beta-hydroxysteroid dehydrogenase is expressed predominantly in the testis and catalyzes the conversion of androstenedione to testosterone. It preferentially uses NADP as cofactor. Deficiency can result in male pseudohermaphroditism with gynecomastia.
Background Reference 1:
Li, J., et al. Breast Cancer Res. 12 (2), R19 (2010)
Background Reference 2:
Sata, F., et al.Sex Med (2010) In press
Background Reference 3:
Ahn, J., et al. Hum. Mol. Genet. 18(19):3749-3757(2009)
Background Reference 4:
Chakrabarti, B., et al. Autism Res 2(3):157-177(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-983

List Size:
400 ul

List Price:

$495.00

Datasheet
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