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Western Blot at 1:1000 dilution + human placenta lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in HL-60 cell line lysates (35ug/lane).
Western blot analysis of HSD3B1 using rabbit polyclonal HSD3B1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HSD3B1 gene.
Western Blot at 1:1000 dilution + human placenta lysate Lysates/proteins at 20 ug per lane.

HSD3B1 Antibody

CATALOG NUMBER: 57-835

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This HSD3B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 26-55 amino acids from the N-terminal region of human HSD3B1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
42 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HSD3B1
Additional Names:
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I, 3-beta-HSD I, Trophoblast antigen FDO161G, 3-beta-hydroxy-Delta(5)-steroid dehydrogenase, 3-beta-hydroxy-5-ene steroid dehydrogenase, Progesterone reductase, Steroid Delta-isomerase, Delta-5-3-ketosteroid isomerase, HSD3B1, 3BH, HSDB3A
Protein Accession Number:
P14060
Protein Gi Number:
112767
Ncbi Gene Id Number:
3283
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. Efficiently catalyzes the transformation of pregnenolone to progesterone, 17-alpha-hydroxypregnenolone to 17-alpha-hydroxyprogesterone, DHEA to 4-androstenedione, dihydrotestosterone to 5-alpha-androstane-3 beta,17 beta-diol, dehydroepiandrosterone to androstenedione and 5-alpha-androstan-3 beta,17 beta-diol to 5-alpha-dihydrotestosterone.
Background Reference 1:
Canzian, F., et al. Hum. Mol. Genet. 19(19):3873-3884(2010)
Background Reference 2:
Shimodaira, M., et al. Eur. J. Endocrinol. 163(4):671-680(2010)
Background Reference 3:
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background Reference 4:
Liu, C.Y., et al. Carcinogenesis 31(7):1259-1263(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-835

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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