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Antibody used in WB on Human 293T at 0.2-1 ug/ml.
Antibody used in IHC on Monkey adrenal gland at: 1:25.
Antibody used in IHC on Monkey vagina at: 1:25.
Antibody used in WB on Human 293T at 0.2-1 ug/ml.

HSD3B2 Antibody

CATALOG NUMBER: 25-940

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HSD3B2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
HSD3B2 antibody can be used for detection of HSD3B2 by ELISA at 1:1562500. HSD3B2 antibody can be used for detection of HSD3B2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
293T Cell Lysate
Predicted Molecular Weight:
42 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store HSD3B2 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
HSD3B2
Additional Names:
HSD3B2, HSDB, HSDB3, HSD3B, SDR11E2
Protein Accession Number:
NP_000189
Protein Gi Number:
4504509
Ncbi Gene Id Number:
3284
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta (5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids.
Background Reference 1:
Shigematsu, K., (2008) Eur. J. Endocrinol. 158 (6), 867-878.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

25-940

List Size:
100 ul

List Price:

$519.00

Datasheet
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