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Western blot analysis of lysed extracts from MCF7 cells using HSF1 (Ab-303).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSF1 (Ab-303).
Western blot analysis of lysed extracts from MCF7 cells using HSF1 (Ab-303).

HSF1 (Ab 303) Antibody

CATALOG NUMBER: 79-385

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
HSF1 (Ab-303) antibody was raised against a peptide sequence around aa.301~305 (P-P-S-P-P) derived from Human HSF1.
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Specificity:
This antibody detects endogenous level of total HSF1 H3.1 protein.
Predicted Molecular Weight:
82 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
HSF1
Additional Names:
HSTF1, CMKBR7, EBI1, EVI1, HSTF 1
Protein Accession Number:
NP_005517.1
Protein Gi Number:
5031767
Ncbi Gene Id Number:
3297
Background:
DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
Background Reference 1:
Parvaneh Rafiee, et, al. (2006) Am J Physiol Cell Physiol ; 291: C931 - C945Fumika Shinozaki, et, al. (2006) J. Biol. Chem ; 281: 16361 - 16369. Eiichi Takaki, et, al. (2006) J. Biol. Chem ; 281: 4931 - 4937. Jan-Jong Hung, et, al. (1998) J. Biol. Chem ; 273: 31924.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-385

List Size:
0.1 mg

List Price:

$370.00

Datasheet
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