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Antibody used in WB on Human Jurkat 0.0625 ug/ml.
Antibody used in WB on Human Jurkat 0.0625 ug/ml.

HSF1 Antibody

CATALOG NUMBER: 31-175

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HSF1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
HSF1 antibody can be used for detection of HSF1 by ELISA at 1:12500. HSF1 antibody can be used for detection of HSF1 by western blot at 0.0625 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Predicted Molecular Weight:
57 kDa, 56 kDa, 56 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store HSF1 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
HSF1
Additional Names:
HSF1, HSTF1
Protein Accession Number:
NP_005517
Protein Gi Number:
5031767
Ncbi Gene Id Number:
3297
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
HSF1 is a heat-shock transcription factor. Transcription of heat-shock genes is rapidly induced after temperature stress. Hsp90, by itself and/or associated with multichaperone complexes, is a major repressor of HSF1.HSF1 is a heat-shock transcription factor. Transcription of heat-shock genes is rapidly induced after temperature stress. Hsp90, by itself and/or associated with multichaperone complexes, is a major repressor of HSF1.
Background Reference 1:
Wang, J., et al., (2006) J. Biol. Chem. 281 (5), 2451-2459.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

31-175

List Size:
100 ul

List Price:

$519.00

Datasheet
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