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Western blot analysis of HSF1 using rabbit polyclonal HSF1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HSF1 gene.
HSF1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis of HSF1 using rabbit polyclonal HSF1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HSF1 gene.

HSF1 Antibody

CATALOG NUMBER: 57-547

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Xenopus
Immunogen:
This HSF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 72-100 amino acids from the N-terminal region of human HSF1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
57 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HSF1
Additional Names:
Heat shock factor protein 1, HSF 1, Heat shock transcription factor 1, HSTF 1, HSF1, HSTF1
Protein Accession Number:
Q00613
Protein Gi Number:
462333
Ncbi Gene Id Number:
3297
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The product of this gene is a heat-shock transcriptionfactor. Transcription of heat-shock genes is rapidly induced aftertemperature stress. Hsp90, by itself and/or associated withmultichaperone complexes, is a major repressor of this gene.
Background Reference 1:
Hayashida, N., et al. EMBO J. 29(20):3459-3469(2010)
Background Reference 2:
Sangle, G.V., et al. Endocrinology 151(9):4455-4466(2010)
Background Reference 3:
Shunmei, E., et al. Mol. Cells 29(5):527-531(2010)
Background Reference 4:
Kim, S.A., et al. Int. J. Oncol. 36(4):867-872(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-547

List Size:
400 ul

List Price:

$495.00

Datasheet
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