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45-750 staining (1ug/ml) of Human Muscle lysate (RIPA buffer, 30ug total protein per lane). Detected by chemiluminescence.
45-750 (10ug/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-750 (10ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-750 staining (1ug/ml) of Human Muscle lysate (RIPA buffer, 30ug total protein per lane). Detected by chemiluminescence.

HSP22 Antibody

CATALOG NUMBER: 45-750

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: NELPQDSQEVTCT
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 20-25kDa band seen in Human Skeletal Muscle lysate [Predicted MW of approx. 22kDa according to NP_055180]. Recommended for use at 0.5-1ug/ml. Primary incubated for 1 hour.Immunohistochemistry:Paraffin embedded Human Adrenal Gland and Tonsil. Recommended concentration: 10ug/ml.
Positive Control 1:
Cat. No. 1375 - Human Skeletal Muscle Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
HSPB8
Additional Names:
HSPB8, heat shock 22kDa protein 8, CMT2L, DHMN2, E2IG1, H11, HMN2, HMN2A, HSP22, Charcot-Marie-Tooth disease, spinal, heat shock 27kDa protein 8, hereditary motor neuropathy, distal, protein kinase H11, small stress protein-like protein HSP22, spinal muscular atrophy, distal, adult, autosomal dominant, E2-induced gene 1, heat shock protein beta-8
Protein Accession Number:
NP_055180.1
Protein Gi Number:
7657146
Ncbi Gene Id Number:
26353
Background Reference 1:
Benndorf R, Sun X, Gilmont RR, Biederman KJ, Molloy MP, Goodmurphy CW, Cheng H, Andrews PC, Welsh MJ. HSP22, a new member of the small heat shock protein superfamily, interacts with mimic of phosphorylated HSP27 ((3D)HSP27). J Biol Chem. 2001 Jul 20;276(29):26753-61.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-750

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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