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Western blot analysis of lysed extracts from K562 and HeLa cells using HSP27 (Ab-82).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP27 (Ab-82).
Western blot analysis of lysed extracts from K562 and HeLa cells using HSP27 (Ab-82).

HSP27 (Ab 82) Antibody

CATALOG NUMBER: 79-379

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
HSP27 (Ab-82) antibody was raised against a peptide sequence around aa. 80~84 (Q-L-S-S-G) derived from Human HSP27.
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Specificity:
This antibody detects endogenous level of total HSP27 protein.
Predicted Molecular Weight:
27 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
HSPB1
Additional Names:
CMT2F, HMN2B, HSP27, HSP28, Hsp25, SRP27, HS.76067, HEL-S-102, NFKB3, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Growth-related 25 kDa protein, HSP 27, HSP25
Protein Accession Number:
NP_001531.1
Protein Gi Number:
4504517
Ncbi Gene Id Number:
3315
Background:
Involved in stress resistance and actin organization.
Background Reference 1:
White MY, et al. (2006) J Mol Cell Cardiol. 40 (6): 761-74Xiankui Sun, et al. (2006) Cell Stress Chaperones. 11 (1): 61–70Matthias Meier; et al. (2001) Hypertension. 38:1260Ali Dana, et al. (2000) Circulation Research. 86: 989

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-379

List Size:
0.1 mg

List Price:

$370.00

Datasheet
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