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Western blot analysis of HSPB1 using rabbit polyclonal HSPB1 Antibody (S78) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HSPB1 gene (Lane 2).
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with HSPB1 Antibody (S78) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of HSPB1 using rabbit polyclonal HSPB1 Antibody (S78) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HSPB1 gene (Lane 2).

HSP27 Antibody

CATALOG NUMBER: 62-721

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This HSP27(HSPB1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 56-85 amino acids from human HSP27(HSPB1).
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
23 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HSPB1
Additional Names:
Heat shock protein beta-1, HspB1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, HSP 27, Stress-responsive protein 27, SRP27, HSPB1, HSP27, HSP28
Protein Accession Number:
P04792
Protein Gi Number:
19855073
Ncbi Gene Id Number:
3315
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock or stress proteins. HSBP1 exhibits rapid increased phosphorylation in response to various mitogens, tumor promoters (e.g. phorbol esters) and calcium ionophores, and high levels are associated with carcinoma of the breast and with endometrial adenocarcinomas. Heat shock of HeLa cell cultures, or treatment with arsenite, phorbol ester, or tumor necrosis factor, causes a rapid phosphorylation of preexisting HSBP1, with Ser82 as the major site and Ser78 the minor site of phosphorylation. HSBP1 may exert phosphorylation-activated functions linked with growth signaling pathways in unstressed cells. A homeostatic function at this level could protect cells from adverse effects of signal transduction systems which may be activated inappropriately during stress.
Background Reference 1:
Wano, C., et al., Exp. Cell Res. 298(2):584-592 (2004).
Background Reference 2:
Evgrafov, O.V., et al., Nat. Genet. 36(6):602-606 (2004).
Background Reference 3:
Song, H., et al., Biochem. Biophys. Res. Commun. 314(1):143-150 (2004).
Background Reference 4:
Chauhan, D., et al., Blood 102(9):3379-3386 (2003).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-721

List Size:
400 ul

List Price:

$495.00

Datasheet
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