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Western blot analysis of lysed extracts from HeLa cells untreated or treated with UV using HSP27 (Phospho-Ser15).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP27 (Phospho-Ser15).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic staining using HSP27 (Phospho-Ser15).
Western blot analysis of lysed extracts from HeLa cells untreated or treated with UV using HSP27 (Phospho-Ser15).

HSP27 (phospho Ser15) Antibody

CATALOG NUMBER: 79-181

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
HSP27 (Phospho-Ser15) antibody was raised against a peptide sequence around phosphorylation site of serine15 (G-P-S (p) -W-D) derived from Human HSP27.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Specificity:
This antibody detects endogenous level of HSP27only when phosphorylated at serine 15.
Predicted Molecular Weight:
27 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
HSPB1
Additional Names:
CMT2F, HMN2B, HSP27, HSP28, Hsp25, SRP27, HS.76067, HEL-S-102, ASPL, RCC17, TUG, UBXD9, UBXN9, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Growth-related 25 kDa protein, HSP 27, HSP25
Protein Accession Number:
NP_001531.1
Protein Gi Number:
4504517
Ncbi Gene Id Number:
3315
Background:
Involved in stress resistance and actin organization.
Background Reference 1:
Tremolada L, et al. (2005) Proteomics. 5 (3): 788-795. McGregor E, et al. (2004) Mol Cell Proteomics. 3 (2): 115-124. Benn SC, et al. (2002) Neuron. 36 (1): 45-56. MacDonald JA, et al. (2002) Mol Cell Proteomics. 1 (4): 314-322.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-181

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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