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Western blot analysis of lysed extracts from HL60 cells untreated or treated with UV using HSP27 (Phospho-Ser78).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP27 (Phospho-Ser78).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic, nuclear, centrosomal, midbody staining using HSP27 (Phospho-Ser78).
Western blot analysis of lysed extracts from HL60 cells untreated or treated with UV using HSP27 (Phospho-Ser78).

HSP27 (phospho Ser78) Antibody

CATALOG NUMBER: 79-216

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
HSP27 (Phospho-Ser78) antibody was raised against a peptide sequence around phosphorylation site of serine 78 (A-L-S (p) -R-Q) derived from Human HSP27.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Specificity:
This antibody detects endogenous level of HSP27 only when phosphorylated at serine 78.
Predicted Molecular Weight:
27 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
HSPB1
Additional Names:
CMT2F, HMN2B, HSP27, HSP28, Hsp25, SRP27, HS.76067, HEL-S-102, KIAA0204, STK2, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Growth-related 25 kDa protein, HSP 27, HSP25
Protein Accession Number:
NP_001531.1
Protein Gi Number:
4504517
Ncbi Gene Id Number:
3315
Background:
Involved in stress resistance and actin organization.
Background Reference 1:
J Landry, et al. (1992) J. Biol. Chem., Vol. 267, Issue 2: 794-803Sita Somara, et al. (2004) Am J Physiol Cell Physiol 286: C1290-C1301Catherine Gaitanaki, et al. (2003) Experimental Biology 206, 2759-2769 (2003) Jing Dong, et al. (2004) Am J Physiol Renal Physiol 287: F1049-F1058

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-216

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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