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43-196 (0.05ug/ml) staining of yeast lysate (wt) and the KO (delta) in th eleft panel and the Ponceau stain in the right panel (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Data obtained by Dr. M. Skoneczny
43-196 (0.05ug/ml) staining of yeast lysate (wt) and the KO (delta) in th eleft panel and the Ponceau stain in the right panel (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Data obtained by Dr. M. Skoneczny

Hsp31p Antibody

CATALOG NUMBER: 43-196

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
S. cerevisiae
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
S. cerevisiae
Immunogen:
The immunogen for this antibody is: C-ETGKFGWDEHSLAKD
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 24kDa band observed in lysates of postdiauxic (overnight grown yeast cells, and no signal in KO grown under identical conditions. Cells grown in logarithmic phase show diminished signals as expected. (calculated MW of 25.7kDa according to NP_010822.1). Recommended concentration: 0.02-0.06ug/ml. This antibody has been successfully used in the following paper: Natkaska et al (2018) PMID: 29264711.
Predicted Molecular Weight:
Approx 24kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
Hsp31p
Additional Names:
Hsp31p, YDR533C
Protein Accession Number:
NP_010822.1
Protein Gi Number:
6320742
Ncbi Gene Id Number:
852146

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-196

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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