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Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: NIH/3T3 whole cell lysate Lane 6: mouse thymus lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: NIH/3T3 whole cell lysate Lane 5: mouse thymus lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: NIH/3T3 whole cell lysate Lane 6: mouse thymus lysate Lysates/proteins at 20 ug per lane.

Hsp60 Antibody

CATALOG NUMBER: 61-328

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This Hsp60 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 340-374 amino acids from the human region of human Hsp60.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:2000
Predicted Molecular Weight:
61 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HSPD1
Additional Names:
60 kDa heat shock protein, mitochondrial, 60 kDa chaperonin, Chaperonin 60, CPN60, Heat shock protein 60, HSP-60, Hsp60, HuCHA60, Mitochondrial matrix protein P1, P60 lymphocyte protein, HSPD1, HSP60
Protein Accession Number:
P10809
Protein Gi Number:
129379
Ncbi Gene Id Number:
3329
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.
Background Reference 1:
Jindal S.,et al.Mol. Cell. Biol. 9:2279-2283(1989).
Background Reference 2:
Venner T.J.,et al.DNA Cell Biol. 9:545-552(1990).
Background Reference 3:
Hansen J.J.,et al.Hum. Genet. 112:71-77(2003).
Background Reference 4:
Tan J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-328

List Size:
200 ul

List Price:

$495.00

Datasheet
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