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Formalin-fixed, paraffin-embedded human bladder carcinoma stained with anti-HSP60 antibody (SPM253)
Formalin-fixed, paraffin-embedded human pancreas stained with anti-HSP60 antibody (SPM253).
Formalin-fixed, paraffin-embedded human bladder carcinoma stained with anti-HSP60 antibody (SPM253)

HSP60 Antibody [SPM253]

CATALOG NUMBER: 33-395

Clonality:
Monoclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Mouse
Species Reactivity:
Bovine, Chicken, Dog, Drosophila, Frog, Hamster, Human, Monkey, Mouse, Pig, Rabbit, Rat, Sheep
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Bovine, Chicken, Dog, Drosophila, Frog, Hamster, Human, Monkey, Mouse, Pig, Rabbit, Rat, Sheep
Immunogen:
Recombinant human protein was used as the immunogen for the anti-HSP60 antibody.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 0.5-1 ug/ml
Western blot: 0.25-0.5 ug/ml
Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)
Prediluted format : incubate for 30 min at RT (2)
Optimal dilution of the anti-HSP60 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG1, kappa
physical-state:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
HSPD1
Additional Names:
60 kDa heat shock protein, mitochondrial, 60 kDa chaperonin, Chaperonin 60, CPN60, Heat shock protein 60, HSP-60, Hsp60, HuCHA60, Mitochondrial matrix protein P1, P60 lymphocyte protein, HSPD1, HSP60
Ncbi Gene Id Number:
3329
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
Recognizes a 60kDa protein, identified as the heat shock protein 60 (Hsp60). Its epitope is localized between amino acids 383-447 of human Hsp60. A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsps), more appropriately called as stress response proteins (srps). Hsp60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial Hsp60, but also against its mammalian homolog.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-395

List Size:
100 ug

List Price:

$626.00

Datasheet
Shipping Info

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