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Western blot analysis of lysates from Wheat using HSP60/HSPD1 Rabbit mAb at 1:10000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 90s.
Western blot analysis of various lysates using HSP60/HSPD1 Rabbit mAb at 1:10000 dilution incubated at room temperature for 1.5 hours. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using HSP60/HSPD1 Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human colon tissue using HSP60/HSPD1 Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using HSP60/HSPD1 Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse intestine tissue using HSP60/HSPD1 Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using HSP60/HSPD1 Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using HSP60/HSPD1 Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Confocal imaging of HeLa cells using HSP60/HSPD1 Rabbit mAb (Red). The cells were counterstained with α-Tubulin Rabbit mAb (Green). DAPI was used for nuclear staining (blue). Objective: 60x.
Immunofluorescence analysis of NIH/3T3 cells using HSP60/HSPD1 Rabbit mAb at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of C6 cells using HSP60/HSPD1 Rabbit mAb at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunoprecipitation of HSP60/HSPD1 from 300 µg extracts of HeLa cells was performed using 2 µg of HSP60/HSPD1 Rabbit mAb. Rabbit Control IgG was used to precipitate the Control IgG sample. IP samples were eluted with 1x Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using HSP60/HSPD1 Rabbit mAb at a dilution of 1:6000.
Western blot analysis of lysates from Wheat using HSP60/HSPD1 Rabbit mAb at 1:10000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 90s.

HSP60/HSPD1 Rabbit Recombinant Antibody (81-041)

CATALOG NUMBER: 81-041

Clonality:
Monoclonal
Tested Applications:
ELISA, IF/ICC, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat, Wheat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat, Wheat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 350-450 of human HSP60/HSPD1 (P10809).
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF/ICC, IHC-P, WB
Application Note:
WB,1:5000 - 1:30000, IHC-P,1:2000 - 1:8000, IF/ICC,1:100 - 1:1000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
Wheat,293F,Mouse liver,Mouse brain,Rat thymus
Predicted Molecular Weight:
61kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
HSPD1
Additional Names:
HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60; HSP60/HSPD1
Protein Accession Number:
P10809
Ncbi Gene Id Number:
3329
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

81-041

List Size:
100 uL

List Price:

$540.00

Datasheet
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