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Western blot analysis of extracts of various cell lines, using HSP70 antibody (13-127) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of U2OS cells using HSP70 antibody (13-127). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using HSP70 antibody (13-127) at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using HSP70 antibody (13-127) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

HSP70 Antibody

CATALOG NUMBER: 13-127

Clonality:
Polyclonal
Tested Applications:
IF, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 540 to the C-terminus of human HSP70 (NP_005336.3).
Conjugate:
Unconjugated
Tested Applications:
IF, IP, WB
Application Note:
WB: 1:500 - 1:2000
IF: 1:50 - 1:200
IP: 1:50 - 1:100
Positive Control 1:
HeLa
Positive Control 2:
NIH/3T3
Positive Control 3:
COS-1
Positive Control 4:
COS-7
Positive Control 5:
U2OS
Positive Control 6:
293T
Predicted Molecular Weight:
Observed: 72kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
HSPA1A
Additional Names:
HSPA1A, HSP72, HSPA1, HSP70I, HSP70-1, HSP70-1A, HEL-S-103
Ncbi Gene Id Number:
3303
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-127

List Size:
100 uL

List Price:

$436.00

Out of stock

Datasheet
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