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Western blot analysis of extracts of various cell lines, using HSP90AA1 antibody (13-479) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Western blot analysis of extracts of various cell lines, using HSP90AA1 antibody (13-479) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

HSP90AA1 Antibody [8G6E1]

CATALOG NUMBER: 13-479

Clonality:
Monoclonal
Tested Applications:
WB
Host Species:
Mouse
Species Reactivity:
Human, Monkey, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human, Monkey, Mouse, Rat
Immunogen:
Recombinant protein of human HSP90AA1
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
WB: 1:1000 - 1:4000
Positive Control 1:
Jurkat
Positive Control 2:
HeLa
Positive Control 3:
293T
Positive Control 4:
MCF7
Positive Control 5:
Mouse brain
Positive Control 6:
Mouse lung
Predicted Molecular Weight:
Observed: 105kDa
Purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
HSP90AA1
Additional Names:
HSP90, HSP90A, HSPC1, HSPCA, Heat shock protein HSP 90-alpha, Heat shock 86 kDa, HSP 86, EL52, HSPN, LAP2, HSP86, Hsp89, Hsp90, HSP89A, HSP90N, HSPCAL1, HSPCAL4
Ncbi Gene Id Number:
3320
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is an inducible molecular chaperone that functions as a homodimer. The encoded protein aids in the proper folding of specific target proteins by use of an ATPase activity that is modulated by co-chaperones. Two transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-479

List Size:
50 uL

List Price:

$335.00

Out of stock

Datasheet
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