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Western blot analysis of lysed extracts from HeLa and A2780 cells using HSP90B (Ab-254).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP90B (Ab-254).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic staining using HSP90B (Ab-254).
Western blot analysis of lysed extracts from HeLa and A2780 cells using HSP90B (Ab-254).

HSP90B (Ab 254) Antibody

CATALOG NUMBER: 79-403

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
HSP90B (Ab-254) antibody was raised against a peptide sequence around aa. 252~256 (V-G-S-D-E) derived from Human HSP90B.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Specificity:
This antibody detects endogenous level of total HSP90B protein.
Predicted Molecular Weight:
92 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
HSP90AB1
Additional Names:
HSP84, HSPC2, HSPCB, D6S182, HSP90B, GYS, HSP 84
Protein Accession Number:
NP_031381.2
Protein Gi Number:
20149594
Ncbi Gene Id Number:
3326
Background:
Molecular chaperone. Has ATPase activity.
Background Reference 1:
Ingrid K. Ruf, et, al. (2005) J. Virol ; 79: 14562 - 14569. Christy M. Hebner, et, al. (2006) J. Gen. Virol ; 87: 3183 - 3193. Rika van Huizen, et, al. (2003) J. Biol. Chem ; 278: 15558 - 15564.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-403

List Size:
0.1 mg

List Price:

$370.00

Datasheet
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