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Western blot analysis of lysed extracts from HeLa cells untreated (Lane 1) or treated with hot shock (lane 2) using HSP90B (Phospho-Ser254).
Western blot analysis of lysed extracts from HeLa cells untreated (Lane 1) or treated with hot shock (lane 2) using HSP90B (Phospho-Ser254).

HSP90B (phospho Ser254) Antibody

CATALOG NUMBER: 79-469

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
HSP90B (Phospho-Ser254) antibody was raised against a peptide sequence around phosphorylation site of serine 254 (V-G-S (p) -D-E) derived from Human HSP90B.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western Blot: 1:500~1:1000
Specificity:
This antibody detects endogenous level of HSP90B only when phosphorylated at serine 254.
Predicted Molecular Weight:
92 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
HSP90AB1
Additional Names:
HSP84, HSPC2, HSPCB, D6S182, HSP90B, TRKB, HS90B, HSP 84, HSP90-beta, HSP90AB1
Protein Accession Number:
NP_031381.2
Protein Gi Number:
20149594
Ncbi Gene Id Number:
3326
Background:
Molecular chaperone. Has ATPase activity.
Background Reference 1:
Zhao R, Houry WA. (2007) Adv Exp Med Biol; 594: 27-36. Olsen JV, et al. (2006) Cell; 127 (3): 635-648Ogiso H, et al. (2004) Biochemistry; 43 (49): 15510-15519

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-469

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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