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Western blot analysis of lysates from C2C12, MCF-7, Hela cell line (from left to right), using HSP90B1 Antibody at 1:1000 at each lane.
HSP90B1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of lysates from C2C12, MCF-7, Hela cell line (from left to right), using HSP90B1 Antibody at 1:1000 at each lane.

HSP90B1 Antibody

CATALOG NUMBER: 56-123

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Monkey
Immunogen:
This HSP90B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 460-487 amino acids from the Central region of human HSP90B1.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
92 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HSP90B1
Additional Names:
Endoplasmin, 94 kDa glucose-regulated protein, GRP-94, Heat shock protein 90 kDa beta member 1, Tumor rejection antigen 1, gp96 homolog, HSP90B1, GRP94, TRA1
Protein Accession Number:
P14625
Protein Gi Number:
119360
Ncbi Gene Id Number:
7184
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
HSP90 proteins are highly conserved molecular chaperonesthat have key roles in signal transduction, protein folding,protein degradation, and morphologic evolution. HSP90 proteinsnormally associate with other cochaperones and play important rolesin folding newly synthesized proteins or stabilizing and refoldingdenatured proteins after stress. HSP90B1 is an endoplasmicreticulum HSP90 protein. Other HSP90 proteins are found in cytosol(see HSP90AA1; MIM 140571) and mitochondria (TRAP1; MIM 606219)(Chen et al., 2005 [PubMed 16269234]).
Background Reference 1:
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background Reference 2:
Suzuki, S., et al. Biochem. Biophys. Res. Commun. 398(3):525-531(2010)
Background Reference 3:
Bloor, S., et al. Proc. Natl. Acad. Sci. U.S.A. 107(15):6970-6975(2010)
Background Reference 4:
Davila, S., et al. Genes Immun. 11(3):232-238(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-123

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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$35 Dry Ice Shipment fee may be required on some items.

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