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Western blot analysis of various lysates using HSP70/HSPA1 Mouse mAb at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 60s.
Western blot analysis of various lysates using HSP70/HSPA1 Mouse mAb at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 60s.

HSPA1A Antibody [1B10F3]

CATALOG NUMBER: 15-858

Clonality:
Monoclonal
Tested Applications:
IHC, WB
Host Species:
Mouse
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human, Mouse, Rat
Immunogen:
A synthetic peptide of human HSPA1A
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:2000 - 1:5000
IHC: 1:50 - 1:200
Positive Control 1:
HepG2
Positive Control 2:
HeLa
Positive Control 3:
A-549
Positive Control 4:
293T
Positive Control 5:
mouse brain
Predicted Molecular Weight:
Observed: 70kDa
Purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
HSPA1A
Additional Names:
HSPA1A, HSP72, HSPA1, HSP70I, HSP70-1, HSP70-1A, HEL-S-103
Ncbi Gene Id Number:
3303
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

15-858

List Size:
100 uL

List Price:

$540.00

Datasheet
Shipping Info

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