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Antibody used in WB on Human 293T at 0.2-1 ug/ml.
Antibody used in WB on Human 293T at 0.2-1 ug/ml.

HSPA4 Antibody

CATALOG NUMBER: 26-101

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HSPA4.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
HSPA4 antibody can be used for detection of HSPA4 by ELISA at 1:62500. HSPA4 antibody can be used for detection of HSPA4 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
293T Cell Lysate
Predicted Molecular Weight:
94 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store HSPA4 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
HSPA4
Additional Names:
HSPA4, APG-2, HS24/P52, MGC131852, RY, hsp70, hsp70RY, HSPH2, HEL-S-5a
Protein Accession Number:
NP_002145
Protein Gi Number:
38327039
Ncbi Gene Id Number:
3308
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
HSPA4 (Hsp70) belongs to the heat shock protein 70 family. It was isolated as a putative Rictor interacting protein and interaction with membranes acts as a platform for its release into the extracellular environment during its recovery from stress. Hsp70 gene expression in Rheumatoid Arthitis-affected synovial tissue is followed by Hsp70 cell surface expression on fibroblast-like synovial cells growing from RA synovial tissue. Serum Hsp70 levels were increased in Systemic sclerosis patients, and associated with pulmonary fibrosis, skin sclerosis, renal vascular damage, oxidative stress, and inflammation.
Background Reference 1:
Kim, H.E., (2008) Mol. Cell 30 (2), 239-247.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

26-101

List Size:
100 ul

List Price:

$519.00

Datasheet
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