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Western blot analysis in A375,A2058,Hela cell line lysates (35ug/lane).
HSPA5 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in A375,A2058,Hela cell line lysates (35ug/lane).

HSPA5 Antibody

CATALOG NUMBER: 61-766

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Pig, Rat, Xenopus
Immunogen:
This HSPA5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 541-568 amino acids from the C-terminal region of human HSPA5.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
72 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HSPA5
Additional Names:
78 kDa glucose-regulated protein, GRP-78, Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78, Heat shock 70 kDa protein 5, Immunoglobulin heavy chain-binding protein, BiP, HSPA5, GRP78
Protein Accession Number:
P11021
Protein Gi Number:
14916999
Ncbi Gene Id Number:
3309
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
When Chinese hamster K12 cells are starved of glucose, thesynthesis of several proteins, called glucose-regulated proteins(GRPs), is markedly increased. Hendershot et al. (1994) [PubMed8020977] pointed out that one of these, GRP78 (HSPA5), alsoreferred to as 'immunoglobulin heavy chain-binding protein' (BiP),is a member of the heat-shock protein-70 (HSP70) family and isinvolved in the folding and assembly of proteins in the endoplasmicreticulum (ER). Because so many ER proteins interact transientlywith GRP78, it may play a key role in monitoring protein transportthrough the cell.
Background Reference 1:
Zhao, C., et al. J. Med. Virol. 82(1):14-22(2010)
Background Reference 2:
Zhuang, L., et al. Mod. Pathol. 23(1):45-53(2010)
Background Reference 3:
Arnaudeau, S., et al. Proteomics 9(23):5316-5327(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-766

List Size:
400 ul

List Price:

$495.00

Datasheet
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