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Western blot analysis of lysate from Jurkat cell line,using HSPA6 Antibody .AP8708b was diluted at 1:1000 at each lane
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with HSPA6 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometry analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of lysate from Jurkat cell line,using HSPA6 Antibody .AP8708b was diluted at 1:1000 at each lane

HSPA6 Antibody

CATALOG NUMBER: 63-604

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This HSPA6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 544-571 amino acids from the C-terminal region of human HSPA6.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
71 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HSPA6
Additional Names:
Heat shock 70 kDa protein 6, Heat shock 70 kDa protein B', HSPA6, HSP70B'
Protein Accession Number:
P17066
Protein Gi Number:
34978357
Ncbi Gene Id Number:
3310
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage.
Background Reference 1:
Leung,T.K., et.al.,Genomics 12 (1), 74-79 (1992)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-604

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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