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45-072 (0.03ug/ml) staining of HepG2 (A) and NIH3T3 (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-072 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (b
45-072 (0.03ug/ml) staining of HepG2 (A) and NIH3T3 (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

HSPA8 Antibody

CATALOG NUMBER: 45-072

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog
Immunogen:
The immunogen for this antibody is: C-EKLQGKINDEDKQK
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 75kDa band observed in lysates of cell lines HepG2 and NIH3T3 (calculated MW of 70.9kDa according to Human NP_006588.1 and Mouse NP_112442.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.03-0.1ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10ug/ml
Specificity:
This antibody is expected to recognise isoform 1 (NP_006588.1) only.
Positive Control 1:
Cat. No. 1202 - A431 Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
HSPA8
Additional Names:
HSPA8 , heat shock 70kDa protein 8 , HSC54, HSC70, HSC71, HSP71, HSP73, HSPA10, LAP, MGC131511, MGC29929, NIP71 , LPS-associated protein 1, N-myristoyltransferase inhibitor protein 71, constitutive heat shock protein 70, heat shock 70kD protein 8, heat shock 70kd protein 10, heat shock cognate protein 54, heat shock cognate protein, 71-kDa, lipopolysaccharide-associated protein , uncharacterized bone marrow protein BM034
Protein Accession Number:
NP_006588.1
Protein Gi Number:
5729877
Ncbi Gene Id Number:
3312
Background Reference 1:
Zhang Z, Fan J, Becker KG, Graff RD, Lee GM, Francomano CA. Comparison of gene expression profile between human chondrons and chondrocytes: a cDNA microarray study. Osteoarthritis Cartilage. 2006 May;14(5):449-59. Epub 2006 Jan 18.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-072

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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