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Western blot analysis of extracts of various cell lines, using HSPA9 antibody (13-203) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat kidney using HSPA9 antibody (13-203) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using HSPA9 antibody (13-203) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using HSPA9 antibody (13-203) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using HSPA9 antibody (13-203) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using HSPA9 antibody (13-203). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using HSPA9 antibody (13-203) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

HSPA9 Antibody

CATALOG NUMBER: 13-203

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 380-679 of human HSPA9 (NP_004125.3).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
MCF7
Positive Control 2:
NIH/3T3
Positive Control 3:
COS-7
Positive Control 4:
Mouse brain
Positive Control 5:
Mouse lung
Predicted Molecular Weight:
Observed: 80kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
HSPA9
Additional Names:
HSPA9, Heat shock 70 kDa protein 9, GRP-75, Heat shock 70kD protein 9B, GRP75, Mortalin-2, Mortalin, perinuclear, Mot-2, MOT2, Peptide-binding protein 74, MOT, MTHSP75, p66-mortalin, PBP74, CSA, HSPA9B, Mortalin
Ncbi Gene Id Number:
3313
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the heat shock protein 70 gene family. The encoded protein is primarily localized to the mitochondria but is also found in the endoplasmic reticulum, plasma membrane and cytoplasmic vesicles. This protein is a heat-shock cognate protein. This protein plays a role in cell proliferation, stress response and maintenance of the mitochondria. A pseudogene of this gene is found on chromosome 2.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-203

List Size:
100 uL

List Price:

$436.00

Datasheet
Shipping Info

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